Lignin-containing cellulose nanofibrils (LCNF) have garnered significant attention in academia and industry due to their high output, low manufacturing costs, and minimal environmental impact [1, 2]. We have developed a TEMPO/laccase/O2 (TLO) approach to oxidize the high lignin content fibers, followed by homogenization to prepare LCNF. The morphology, nanostructure, and residual lignin content of LCNF could be tailored by controlling the TLO oxidation time, enabling the regulation of its crystallinity, hydrophobicity, and rheological properties. As the oxidation time increased from 24 h to 96 h, the carboxylate content of LCNF gradually increased from 0.3 to 1.1 mmol/g, while the lignin content of LCNF decreased from 30.1% to 20.7%. The results of SEM, TEM, UV-Vis, and FTIR confirmed the presence of lignin in LCNF, demonstrating that fibrous network structures coexisted with lignin. The obtained oxidized LCNF showed higher emulsification ability for stabilizing a Pickering emulsion when compared to unoxidized LCNF. More importantly, the Pickering emulsion stabilized with oxidized LCNF exhibited long-term emulsion stability (over 7 days) with a relatively narrow distribution of droplet size around 3.8 – 7.6 µm. This work presents a green pathway for achieving sustainable production of LCNF with high yeild and provides practical guidance for potential applications of LCNF-based Pickering emulsions with untapped potential.