The outstanding mechanical properties of cellulose have long motivated us to use it as a sustainable source to improve the mechanical properties of materials. After isolating cellulose from wood pulp and defibrillating it into nanocellulose, the reported mechanical stiffness ranges between 110-150 GPa.[1, 2] Since 2014, a spinning process utilising a flow-focusing microfluidic device that aligns and structures nanofibers into continuous filaments has been developed.[3] This process led to the production of the strongest bio-based filaments, with a reported stiffness of 86 GPa.[4] To provide a basis for upscaling of the spinning process, we have investigated the effect of elevated drying temperatures (60 °C, 105 °C, and 160 °C) on filament mechanical properties, comparing them to filaments dried at ambient conditions (20 °C). We observed a decrease in mechanical performance up to 105 °C, followed by an increase at 160 °C. To understand the underlying structural changes responsible for this trend, we analysed the filaments using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Atomic Force Microscopy (AFM) and correlated the findings with the observed mechanical behaviour.