NFC production is a well-known process that is more and more used to produce high added-value products for many applications such as food packaging, rheology agents or composites. However, production requires a lot of energy such as electricity or water. Starting from industrial poplar wood chips, we propose to combine an enzymatic treatment with a mechanic treatment in a Modular Screw Device (MSD) to fragilize their structure. This pretreatment allows to produce Micro/Nanofibrillated Cellulose (M/NFC) from the obtained pulps with reduced energy consumption and enhanced defibrillation of materials. In this study, we selected different enzymes, used alone or in cocktail, to target the three main components of wood biomass: Endoglucanases will break β-1-4 bonds in cellulose structure that can facilitate fibrils separation and cellulose microfibrillation; Xylanases will target xylans from hemicellulose and weakens the fiber structure; and laccases will degrade lignin, facilitating delignification during cooking process at different delignification level linked to the cooking condition (source of alkali, duration). Following the selected cooking, we obtained fibers that have been bleached with a Totally Chlorine Free (TCF) bleaching sequence. According to this process, we produced fiber suspensions or different pulps with different lignin levels. Starting from these materials, M/NFC suspensions have been produced with several passes in a high pressure homogenizer and energy consumption has been monitored. The produced M/NFC were characterized by state-of-the-art techniques to obtain the quality index of nanomaterials. To do so, eight characterizations were performed on the suspension: size microparticle, homogeneity, nanosized fraction and turbidity. Moreover, nanopapers were produced at lab scale to determine, transmittance, tear resistance, Young’s Modulus and porosity. Then, the suspensions were evaluated for their stabilization properties in pickering emulsions depending on the particle size and geometry.